The Human Tyrosine Kinase 2 (TYK2) ELISA Kit is a cutting-edge tool for the precise measurement of TYK2 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable asset for various research endeavors.TYK2 is a critical enzyme that plays a pivotal role in the regulation of immune responses, cell growth, and inflammation. Dysregulation of TYK2 has been implicated in various autoimmune disorders, cancer, and inflammatory diseases, underscoring its importance as a potential therapeutic target and diagnostic marker.
By utilizing the Human TYK2 ELISA Kit, researchers can gain valuable insights into the role of TYK2 in disease pathogenesis, paving the way for the development of novel treatment strategies and personalized medicine approaches. Trust in this advanced ELISA kit to drive your research forward and unlock new possibilities in the field of molecular biology.
Product Name:
Human Tyrosine Kinase 2 (Tyk2) ELISA Kit
Product Code:
HUDL03024
Size:
96 Assays
Target:
Tyk2
Synonyms:
JTK1, Non-Receptor Tyrosine-Protein Kinase TYK2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
89-99
94
Heparin plasma (n=5)
90-98
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
