Human Tyrosine Kinase, Non Receptor 2 (TNK2) ELISA Kit
The Human Tyrosine Kinase Non-Receptor 2 (TNK2) ELISA Kit is a powerful tool for quantitative measurement of TNK2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is specifically designed to provide accurate and reliable results, with high sensitivity and specificity.TNK2 is an important enzyme that plays a key role in cell signaling pathways, regulating processes such as cell growth, proliferation, and survival.
Dysregulation of TNK2 has been linked to various diseases including cancer, making it a valuable biomarker for research and therapeutic development.With the Human TNK2 ELISA Kit, researchers can confidently study the role of TNK2 in disease progression and explore its potential as a target for novel treatments. Get precise and consistent results with this advanced ELISA kit from Assay Genie.
Product Name:
Human Tyrosine Kinase, Non Receptor 2 (TNK2) ELISA Kit
Product Code:
HUDL02944
Size:
96 Assays
Target:
TNK2
Synonyms:
ACK, ACK1, Activated CDC42 Associated Kinase 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
