Description
Human U2AF1 Recombinant Protein (N-His)
Human U2AF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Arg196, with an N-terminal His tag. Predicted molecular weight: 25.18 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Splicing factor U2AF 35 kDa subunit (U2AF1) is a ~27 kDa protein. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. For research use only.
Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
| Product Name: | Human U2AF1 Recombinant Protein (N-His) |
| SKU: | GXRP04451 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | U2AFBP, U2 auxiliary factor 35 kDa subunit, U2AF1, U2 snRNP auxiliary factor small subunit, U2 small nuclear RNA auxiliary factor 1, Splicing factor U2AF 35 kDa subunit, U2AF35 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Arg196 |
| Calculated MW: | 25.18 kDa |
| UniProt: | Q01081 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
