Description
Human UBE2D3 Recombinant Protein (N-His)
Human UBE2D3 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Met147, with an N-terminal His tag. Predicted molecular weight: 18.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. For research use only.
In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin.
| Product Name: | Human UBE2D3 Recombinant Protein (N-His) |
| SKU: | GXRP05033 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Met147 |
| Calculated MW: | 18.85 kDa |
| UniProt: | P61077 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
