Description
Human UBE3A Recombinant Protein (N-His)
Human UBE3A Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met291-Leu407, with an N-terminal His tag. Predicted molecular weight: 15.61 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin-protein ligase E3A (UBE3A) is a ~100 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. For research use only.
Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation.
| Product Name: | Human UBE3A Recombinant Protein (N-His) |
| SKU: | GXRP02054 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Oncogenic protein-associated protein E6-AP, Ubiquitin-protein ligase E3A, UBE3A, E6AP ubiquitin-protein ligase, HPVE6A, EPVE6AP, HECT-type ubiquitin transferase E3A, E6AP, Human papillomavirus E6-associated protein, Renal carcinoma antigen NY-REN-54 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met291-Leu407 |
| Calculated MW: | 15.61 kDa |
| UniProt: | Q05086 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
