Description
Human UBE3C Recombinant Protein (N-His)
Human UBE3C Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Phe719-Ser1083, with an N-terminal His tag. Predicted molecular weight: 43.64 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin-protein ligase E3C is a ~123 kDa protein. E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains. For research use only.
Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome. Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins. Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation.
| Product Name: | Human UBE3C Recombinant Protein (N-His) |
| SKU: | GXRP11516 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:2.3.2.26, HECT-type ubiquitin transferase E3C, HectH2, Homologous to E6AP carboxyl terminus homologous protein 2, KIAA0010, KIAA10, RAUL, RTA-associated ubiquitin ligase, UBE3C, Ubiquitin-protein ligase E3C |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Phe719-Ser1083 |
| Calculated MW: | 43.64 kDa |
| UniProt: | Q15386 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
