Human UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) ELISA Kit
The Human UDP Glucuronosyltransferase 2 Family Polypeptide B7 (UGT2B7) ELISA Kit is specifically designed for the precise quantification of UGT2B7 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.UGT2B7 is a vital enzyme involved in the metabolism and elimination of numerous drugs and toxins in the body. Variations in UGT2B7 activity can impact drug efficacy, toxicity, and overall patient outcomes.
As such, measuring UGT2B7 levels is crucial for understanding drug metabolism pathways and individualizing medication regimens for optimal therapeutic benefits.With its ability to provide reliable data on UGT2B7 expression levels, the UGT2B7 ELISA Kit holds great potential for advancing research in pharmacogenomics, personalized medicine, and drug development. Invest in this innovative kit to enhance your studies on drug metabolism and its implications on patient care.
Product Name:
Human UDP Glucuronosyltransferase 2 Family, Polypeptide B7 (UGT2B7) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
