Description
Human UHRF1 Recombinant Protein (N-His)
Human UHRF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly427-Gly636, with an N-terminal His tag. Predicted molecular weight: 26.00 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. E3 ubiquitin-protein ligase UHRF1 is a ~89 kDa protein. E3 ubiquitin-protein ligase that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. For research use only.
Plays a key role in DNA methylation inheritance by promoting recruitment of DNMT1 to hemimethylated DNA and ensure faithful propagation of the DNA methylation patterns through DNA replication. Acts both as a histone reader and writer: specifically recognizes and binds (1) hemimethylated DNA at replication forks and (2) histone H3 trimethylated at 'Lys-9' and unmethylated at 'Arg-2' (H3K9me3 and H3R2me0, respectively), thereby activating its E3 ubiquitin-protein ligase activity. UHRF1 then mediates histone H3 'Lys-18' monoubiquitination (H3K18ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance. Also mediates histone H3 'Lys-14' and 'Lys-23' ubiquitination (H3K14ub and H3K23ub) at lower level.
| Product Name: | Human UHRF1 Recombinant Protein (N-His) |
| SKU: | GXRP04291 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Nuclear zinc finger protein Np95, RING-type E3 ubiquitin transferase UHRF1, RING finger protein 106, Transcription factor ICBP90, E3 ubiquitin-protein ligase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, NP95, HuNp95, Nuclear protein 95, RNF106, ICBP90, hNp95, UHRF1, Inverted CCAAT box-binding protein of 90 kDa, hUHRF1, Ubiquitin-like-containing PHD and RING finger domains protein 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly427-Gly636 |
| Calculated MW: | 26.00 kDa |
| UniProt: | Q96T88 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
