Description
Human ULK1 Recombinant Protein (N-His)
Human ULK1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-His275, with an N-terminal His tag. Predicted molecular weight: 33.40 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Serine/threonine-protein kinase ULK1 is a ~112 kDa protein. Serine/threonine-protein kinase involved in autophagy in response to starvation. For research use only.
Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences.
| Product Name: | Human ULK1 Recombinant Protein (N-His) |
| SKU: | GXRP08174 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, KIAA0722, Serine/threonine-protein kinase ULK1, hATG1, ATG1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-His275 |
| Calculated MW: | 33.40 kDa |
| UniProt: | O75385 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
