Human Uncoupling Protein 2, Mitochondrial (UCP2) ELISA Kit
The Human Uncoupling Protein 2 Mitochondrial (UCP2) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of UCP2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Uncoupling protein 2 (UCP2) is a mitochondrial protein that regulates energy metabolism and is involved in the control of reactive oxygen species production. It plays a crucial role in various physiological and pathological processes, making it a valuable biomarker for studying metabolic disorders, obesity, and mitochondrial dysfunction.
By utilizing the Human UCP2 ELISA Kit, researchers can gain valuable insights into the role of UCP2 in various diseases and metabolic processes, ultimately leading to a better understanding of potential therapeutic targets and interventions. Trust Assay Genie to provide you with high-quality assays for your research needs.
Product Name:
Human Uncoupling Protein 2, Mitochondrial (UCP2) ELISA Kit
Product Code:
HUDL03044
Size:
96 Assays
Target:
UCP2
Synonyms:
SLC25A8, UCPH, Solute carrier family 25 member 8
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
