Description
Human UPF1 Recombinant Protein (N-His)
Human UPF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Val292-Gln710, with an N-terminal His tag. Predicted molecular weight: 49.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Regulator of nonsense transcripts 1 (UPF1) is a ~124 kDa protein. RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. For research use only.
Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors.
| Product Name: | Human UPF1 Recombinant Protein (N-His) |
| SKU: | GXRP04538 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | hUpf1, Nonsense mRNA reducing factor 1, UPF1, Regulator of nonsense transcripts 1, RENT1, KIAA0221, ATP-dependent helicase RENT1, NORF1, Up-frameshift suppressor 1 homolog |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Val292-Gln710 |
| Calculated MW: | 49.16 kDa |
| UniProt: | Q92900 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
