Description
Human USP53 Recombinant Protein (N-His)
Human USP53 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ala27-Cys380, with an N-terminal His tag. Predicted molecular weight: 42.73 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Ubiquitin carboxyl-terminal hydrolase 53 is a ~120 kDa protein. Deubiquitinase that mediates 'Lys-63'-linked deubiquitination of tight junction proteins, such as MARVELD2 and LSR, and which is involved in the survival of auditory hair cells and hearing. For research use only.
Specifically cleaves 'Lys-63'-linked polyubiquitin chains composed of at least 3 ubiquitin molecules, while it is not able to deubiquitinate substrates with shorter ubiquitin chains: recognizes ubiquitin chain in position S2 and catalyzes en bloc cleavage of polyubiquitin chains from substrate proteins. Probably acts by modulating the barrier properties and mechanical stability of tight junctions via deubiquitination of MARVELD2 and LSR.
| Product Name: | Human USP53 Recombinant Protein (N-His) |
| SKU: | GXRP11609 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | EC:3.4.19.12, KIAA1350, USP53, Ubiquitin carboxyl-terminal hydrolase 53, Ubiquitin-specific peptidase 53 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ala27-Cys380 |
| Calculated MW: | 42.73 kDa |
| UniProt: | Q70EK8 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
