The Human VF (Visfatin) ELISA Kit is specifically designed for the precise measurement of visfatin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Visfatin is an important protein that plays a key role in regulating glucose metabolism and insulin sensitivity. It is also involved in inflammation and immune response, making it a valuable biomarker for studying metabolic disorders, autoimmune diseases, and inflammatory conditions. With its advanced technology and reliable performance, the Human VF (Visfatin) ELISA Kit is a valuable tool for researchers and clinicians working on understanding the role of visfatin in various diseases and developing potential therapeutic interventions.
Product Name:
Human VF (Visfatin) ELISA Kit
SKU:
HUES02741
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
