The Human VIM (Vimentin) ELISA Kit is a powerful tool for accurately detecting vimentin levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results that are suitable for a variety of research applications.Vimentin is an important protein involved in maintaining cellular structure and integrity, playing a key role in cell migration and signaling pathways. Abnormal vimentin expression is associated with various diseases including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for disease progression and therapeutic interventions.With its reliable performance and versatility, the Human VIM (Vimentin) ELISA Kit is an essential tool for researchers studying vimentin biology and its implications in various health conditions.
Product Name:
Human VIM (Vimentin) ELISA Kit
SKU:
HUES02188
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
