The Human Vitamin D Receptor (VDR) ELISA Kit is a reliable and accurate tool for measuring levels of vitamin D receptor in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for various research applications.The vitamin D receptor plays a crucial role in regulating the effects of vitamin D on gene expression, cell growth, and immune function. It is associated with a wide range of health conditions, including bone health, autoimmune diseases, and cancer.
Understanding the levels of vitamin D receptor in the body is essential for studying its impact on these diseases and developing targeted therapies.With the Human VDR ELISA Kit, researchers can easily quantify vitamin D receptor levels in biological samples, enabling more in-depth studies on the role of vitamin D in disease processes and personalized medicine approaches. Trust assaygenie.com for high-quality ELISA kits that provide accurate and reliable results for your research needs.
Product Name:
Human Vitamin D Receptor (VDR) ELISA Kit
Product Code:
HUDL03080
Size:
96 Assays
Target:
VDR
Synonyms:
NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
