The Human VDR (Vitamin D Receptor) ELISA Kit is specifically designed for the precise measurement of Vitamin D receptor levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, ensuring dependable and reproducible results for various research purposes.The Vitamin D receptor is a key protein that plays a vital role in regulating the effects of Vitamin D in the body, impacting various biological processes such as calcium metabolism, immune function, and cell growth. Dysregulation of the Vitamin D receptor has been associated with numerous health conditions, including osteoporosis, autoimmune diseases, and certain cancers, making it an essential marker for studying these diseases and potential therapeutic interventions.Overall, the Human VDR ELISA Kit from AssayGenie provides researchers with a powerful tool for investigating the role of Vitamin D receptor in health and disease, offering valuable insights into its mechanisms and potential clinical implications.
Product Name:
Human VDR (Vitamin D Receptor) ELISA Kit
SKU:
HUES02964
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
