Description
Human VPS4B Recombinant Protein (N-His)
Human VPS4B Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gly138-Gly444, with an N-terminal His tag. Predicted molecular weight: 36.25 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Vacuolar protein sorting-associated protein 4B (VPS4B) is a ~49 kDa protein. Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. For research use only.
Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.
| Product Name: | Human VPS4B Recombinant Protein (N-His) |
| SKU: | GXRP04984 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | SKD1, VPS4B, Suppressor of K(+) transport growth defect 1, Cell migration-inducing gene 1 protein, Protein SKD1, Vacuolar protein sorting-associated protein 4B, VPS42 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gly138-Gly444 |
| Calculated MW: | 36.25 kDa |
| UniProt: | O75351 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
