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Key Features

Human VSTM1 ColorStep ELISA Kit (AEFI02523)

Datasheet MSDS
SKU AEFI02523
Product Type ELISA Kit
Size 96T
Reactivity Human
Detection Method Sandwich ELISA, Double Antibody
Sensitivity 18.75pg/ml
Range 31.25-2000pg/ml
Detection Wavelength OD450
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human VSTM1 ColorStep ELISA Kit
SKU:AEFI02523
Size:96T
Reactivity:Human
Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Detection Method:Sandwich ELISA, Double Antibody
Detection Wavelength:OD450
Reaction Duration:120 minutes
Synonyms:V-set and transmembrane domain-containing protein 1 ELISA Kit, Signal inhibitory receptor on leukocytes-1 ELISA Kit, SIRL-1 ELISA Kit, VSTM1 ELISA Kit
Kit Components:
ComponentsQuantityStorage
48T96T
ELISA
Microplate(Dismountable)		8×68×12Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
Lyophilized Standard1vial2vialPut the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
Cap/Det Ab
(Ready to use, blue)		3ml6ml2-8°C (Avoid Direct Light)
HRP-Streptavidin
(Ready to use, orange)		5ml10ml
TMB Substrate5ml10ml
Sample Dilution Buffer20ml20ml2-8°C
Stop Solution5ml5ml
Wash Buffer(25X)15ml30ml
Plate Sealer3 pieces5 pieces
Manual1 copy1 copy

Other Materials Required (Not provided)

  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water

This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody c