Description
Human WRN Recombinant Protein (N-His)
Human WRN Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn533-Asp944, with an N-terminal His tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN is a ~162 kDa protein. Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity on partially duplex substrates. For research use only.
Also has 3'->5' exonuclease activity towards double-stranded (ds)DNA with a 5'-overhang. Has no nuclease activity towards single-stranded (ss)DNA or blunt-ended dsDNA. Helicase activity is most efficient with (d)ATP, but (d)CTP will substitute with reduced efficiency; strand displacement is enhanced by single-strand binding-protein (heterotrimeric replication protein A complex, RPA1, RPA2, RPA3). Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions.
| Product Name: | Human WRN Recombinant Protein (N-His) |
| SKU: | GXRP13508 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 3'-5' exonuclease, ATP-dependent helicase, Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN, DNA 3'-5' helicase WRN, DNA helicase, RecQ-like type 3, EC:3.1.-.-, EC:5.6.2.4, RECQ3, RECQL2, RecQ protein-like 2, WRN, Werner syndrome protein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn533-Asp944 |
| UniProt: | Q14191 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
