Human Xaa-Pro Dipeptidase/Prolidase/PEPD ELISA Kit
The Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit is a reliable and sensitive assay designed for the accurate measurement of PEPD levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and reproducible results for various research applications. PEPD, also known as Proline Dipeptidase, plays a critical role in the breakdown of peptides containing proline residues, contributing to various biological processes including collagen turnover, wound healing, and immune response regulation. Dysregulation of PEPD activity has been implicated in conditions such as fibrosis, inflammatory disorders, and connective tissue diseases, highlighting its importance as a potential biomarker for disease research and drug development.
By utilizing the Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit, researchers can accurately quantify PEPD levels in biological samples, providing valuable insights into the function and regulation of this enzyme in physiological and pathological settings. With its robust performance and ease of use, this kit is a valuable tool for studying PEPD-related pathways and developing novel therapeutic strategies.
Product Name:
Human Xaa-Pro Dipeptidase/Prolidase/PEPD ELISA Kit
SKU:
HUFI02093
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
1.875 ng/mL
Range:
3.125-200 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-103%
91-101%
80-95%
EDTA Plasma (n = 5)
86-101%
82-101%
81-91%
Heparin Plasma (n = 5)
88-104%
90-96%
86-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
94-105
99
EDTA Plasma (n = 5)
86-105
95
Heparin Plasma (n = 5)
88-99
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
