Description
Human XPA Recombinant Protein (N-His)
Human XPA Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp101-Met273, with an N-terminal His tag. Predicted molecular weight: 23.16 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA repair protein complementing XP-A cells is a ~31 kDa protein. Involved in DNA nucleotide excision repair (NER). For research use only.
Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. During NER stimulates the 5'-3' helicase activity of XPD/ERCC2 and the DNA translocase activity of XPB/ERCC3. Connects XPD/ERCC2 and XPB/ERCC3 during NER, retaining DNA near the XPB/ERCC3 active site, and stabilizing the complex in a different conformation than in transcribing TFIIH.
| Product Name: | Human XPA Recombinant Protein (N-His) |
| SKU: | GXRP09135 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNA repair protein complementing XP-A cells; Xeroderma pigmentosum group A-complementing protein; XPA; XPAC |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp101-Met273 |
| Calculated MW: | 23.16 kDa |
| UniProt: | P23025 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
