Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep) (GXRP13853)

Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep)

Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep) is a recombinant protein expressed in E. coli, spanning Ser334-Ser416, with an N-terminal His-SUMO tag and a C-terminal Strep tag. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. YTH domain-containing protein 1 is a ~84 kDa protein. Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. For research use only.

M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.