The Human Zonulin ELISA Kit is a highly reliable tool for the precise measurement of zonulin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it well-suited for various research purposes.Zonulin is a key protein in regulating the permeability of the intestinal epithelial barrier. Dysregulation of zonulin has been associated with various gastrointestinal disorders, autoimmune diseases, and metabolic conditions, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions. With its advanced technology and user-friendly protocol, the Human Zonulin ELISA Kit is an indispensable tool for researchers seeking to deepen their understanding of zonulin biology and its implications in health and disease.
Product Name:
Human Zonulin ELISA Kit
SKU:
HUES03536
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
85-101
98-109
94-105
Average (%)
93
105
101
1:4
Range (%)
84-96
100-109
91-100
Average (%)
90
105
95
1:8
Range (%)
94-103
91-103
88-99
Average (%)
100
97
93
1:16
Range (%)
96-110
93-102
92-104
Average (%)
104
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
87-99
91
Cell Culture Media (n=8)
91-99
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.37
5.99
20.46
2.22
6.28
22.07
Standard deviation
0.15
0.4
1.03
0.16
0.43
1.46
C V (%)
6.4
6.65
5.01
7.2
6.81
6.62
Uniport ID:
P00738
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human Zonulin in samples.No significant cross-reactivity or interference between Human Zonulin and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Cardiovascular
Le et al.
Development of an Inflammation-Triggered In Vitro “Leaky Gut” Model Using Caco-2/HT29-MTX-E12 Combined with Macrophage-like THP-1 Cells or Primary Human-Derived Macrophages
Anti-Salmonella Defence and Intestinal Homeostatic Maintenance In Vitro of a Consortium Containing Limosilactobacillus fermentum 3872 and Ligilactobacillus salivarius 7247 Strains in Human, Porcine, and Chicken Enterocytes
