IBA57 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
IBA57 Antibody
SKU:
PACO60092
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human, Mouse
Immunogen:
Recombinant Human Putative transferase CAF17, mitochondrial protein (286-356AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
Q5T440
Form:
Liquid
Tested Applications:
ELISAWBIHCIF
Recommended Dilution:
Application
Recommended Dilution
WB
1:500-1:5000
IHC
1:200-1:500
IF
1:50-1:200
Synonyms:
C1orf69 antibody, CAF17_HUMAN antibody, Chromosome 1 open reading frame 69 antibody, FLJ12734 antibody, FLJ13849 antibody, IBA57 antibody, IBA57 homolog iron sulfur cluster assembly antibody, IBA57 iron sulfur cluster assembly homolog antibody, IBA57 iron sulfur cluster assembly homolog (S. cerevisiae) antibody, IBA57, S. cerevisiae, homolog of antibody, Iron sulfur cluster assembly factor for biotin synthase and aconitase like antibody, iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins, with a mass of 57kDa antibody, Iron-sulfur cluster assem...Read more
Western Blot Positive WB detected in: MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, Mouse liver tissue All lanes: IBA57 antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 39 kDa
IHC image of PACO60092 diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO60092 diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of PC-3 cells with PACO60092 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
