The ICOS/CD278 Monoclonal Antibody (CAB5130) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbit monoclonal technology, is optimized for detection of ICOS protein in human samples and is validated for use in various applications such as Western blot, immunohistochemistry, and flow cytometry.ICOS is a crucial co-stimulatory molecule expressed on activated T cells and plays a key role in regulating T cell function and immune responses. Dysregulation of ICOS signaling has been implicated in various autoimmune disorders, allergies, and cancer, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ICOS/CD278 Monoclonal Antibody
SKU:
CAB5130
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1148
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AILIM, CD278, CVID1, ICOS/CD278
Positive Sample:
HL-60, Mouse thymus, Mouse spleen, Rat thymus, Rat spleen
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
23kDa
Observed MW:
22kDa
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Purification Method
Affinity purification
Gene ID
29851
RRID
AB_2863458
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ICOS/CD278 Rabbit mAb (CAB5130) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
