IFT172 Antibody (PACO59501)
- SKU:
- PACO59501
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | IFT172 Antibody (PACO59501) |
| Antibody SKU: | PACO59501 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Intraflagellar transport protein 172 homolog protein (1368-1502AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO59501 diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO59501 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59501 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Required for the maintenance and formation of cilia. Plays an indirect role in hedgehog (Hh) signaling, cilia being required for all activity of the hedgehog pathway (By similarity). |
| Synonyms: | Intraflagellar transport protein 172 homolog, IFT172, KIAA1179 |
| UniProt Protein Function: | IFT172: Required for the maintenance and formation of cilia. Plays an indirect role in hedgehog (Hh) signaling, cilia being required for all activity of the hedgehog pathway. Belongs to the IFT172 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 2p23.3Cellular Component: axoneme; vesicle; ciliumBiological Process: limb development; epidermis development; Notch signaling pathway; smoothened signaling pathway; organelle organization and biogenesis; palate development; spinal cord motor neuron differentiation; cytoplasmic microtubule organization and biogenesis; dorsal/ventral pattern formation; neural tube closure; positive regulation of smoothened signaling pathway; cilium biogenesis; protein processing; heart looping; brain development; negative regulation of epithelial cell proliferationDisease: Short-rib Thoracic Dysplasia 10 With Or Without Polydactyly; Retinitis Pigmentosa 71 |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9UG01 |
| NCBI GenInfo Identifier: | 46358428 |
| NCBI Gene ID: | 26160 |
| NCBI Accession: | NP_056477.1 |
| UniProt Secondary Accession: | Q9UG01,Q86X44, Q96HW4, Q9UFJ9, Q9ULP1, A5PKZ0, B2RNU5 |
| UniProt Related Accession: | Q9UG01 |
| Molecular Weight: | 197,576 Da |
| NCBI Full Name: | intraflagellar transport protein 172 homolog |
| NCBI Synonym Full Names: | intraflagellar transport 172 |
| NCBI Official Symbol: | IFT172Â Â |
| NCBI Official Synonym Symbols: | SLB; wim; osm-1; NPHP17; SRTD10Â Â |
| NCBI Protein Information: | intraflagellar transport protein 172 homolog; wimple homolog; intraflagellar transport 172 homolog; selective LIM binding factor homolog |
| UniProt Protein Name: | Intraflagellar transport protein 172 homolog |
| UniProt Synonym Protein Names: | |
| Protein Family: | Intraflagellar transport protein |
| UniProt Gene Name: | IFT172Â Â |
| UniProt Entry Name: | IF172_HUMAN |