Anti-IGFBP4 Antibody (CAB2008)

Antibody Name:	Anti-IGFBP4 Antibody
Antibody SKU:	CAB2008
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-258 of human IGFBP4 (NP_001543.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-258 of human IGFBP4 (NP_001543.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DEAI HCPP CSEE KLAR CRPP VGCE ELVR EPGC GCCA TCAL GLGM PCGV YTPR CGSG LRCY PPRG VEKP LHTL MHGQ GVCM ELAE IEAI QESL QPSD KDEG DHPN NSFS PCSA HDRR CLQK HFAK IRDR STSG GKMK VNGA PRED ARPV PQGS CQSE LHRA LERL AASQ SRTH EDLY IIPI PNCD RNGN FHPK QCHP ALDG QRGK CWCV DRKT GVKL PGGL EPKG ELDC HQLA DSFR E
Gene ID:	3487
Uniprot:	P22692
Cellular Location:	Secreted
Calculated MW:	17kDa/27kDa
Observed MW:	17kDa

Synonyms:

IGFBP4, BP-4, HT29-IGFBP, IBP4, IGFBP-4

Background:

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

UniProt Protein Function:	IGFBP4: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: extracellular region; extracellular space Molecular Function:insulin-like growth factor I binding; insulin-like growth factor II binding; receptor binding Biological Process: cell proliferation; cellular protein metabolic process; DNA metabolic process; regulation of insulin-like growth factor receptor signaling pathway; signal transduction; skeletal development
NCBI Summary:	This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]
UniProt Code:	P22692
NCBI GenInfo Identifier:	124065
NCBI Gene ID:	3487
NCBI Accession:	P22692.2
UniProt Secondary Accession:	P22692,Q5U012, Q9UCL6, A0N9W2, B4E351,
UniProt Related Accession:	P22692
Molecular Weight:	17,635 Da
NCBI Full Name:	Insulin-like growth factor-binding protein 4
NCBI Synonym Full Names:	insulin like growth factor binding protein 4
NCBI Official Symbol:	IGFBP4
NCBI Official Synonym Symbols:	BP-4; IBP4; IGFBP-4; HT29-IGFBP
NCBI Protein Information:	insulin-like growth factor-binding protein 4
UniProt Protein Name:	Insulin-like growth factor-binding protein 4
Protein Family:	Insulin-like growth factor-binding protein
UniProt Gene Name:	IGFBP4
UniProt Entry Name:	IBP4_HUMAN

	Western blot analysis of extracts of various cell lines, using IGFBP4 antibody (CAB2008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded rat brain using IGFBP4 Antibody (CAB2008) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using IGFBP4 Antibody (CAB2008) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using IGFBP4 Antibody (CAB2008) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using IGFBP4 antibody (CAB2008) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-IGFBP4 Antibody (CAB15068)	Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) CLIA Kit (HUES00321)
	Human IGFBP-4 (Insulin Like Growth Factor Binding Protein 4) ELISA Kit (HUES01656)
	Human Insulin-like growth factor-binding protein 4 (IGFBP4) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)