Anti-IKKGamma Antibody (CAB0917)

Antibody Name:	Anti-IKKGamma Antibody
Antibody SKU:	CAB0917
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKKGamma (NP_003630.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human IKKGamma (NP_003630.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNRH LWKS QLCE MVQP SGGP AADQ DVLG EESP LGKP AMLH LPSE QGAP ETLQ RCLE ENQE LRDA IRQS NQIL RERC EELL HFQA SQRE EKEF LMCK FQEA RKLV ERLG LEKL DLKR QKEQ ALRE VEHL KRCQ QQMA EDKA SVKA QVTS LLGE LQES QSRL EAAT KECQ ALEG RARA ASEQ ARQL ESER EALQ QQHS VQVD QLRM QGQS VEAA LRME RQAA SEEK RKLA QLQV AYHQ LFQE YDNH IKSS VVGS ERKR GMQL EDLK QQLQ QAEE ALVA KQEV IDKL KEEA EQHK IVME TVPV LKAQ ADIY KADF QAER QARE KLAE KKEL LQEQ LEQL QREY SKLK ASCQ ESAR IEDM RKRH VEVS QAPL PPAP AYLS SPLA LPSQ RRSP PEEP PDFC CPKC QYQA PDMD TLQI HVME CIE
Gene ID:	8517
Uniprot:	Q9Y6K9
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	36kDa/48kDa/55kDa
Observed MW:	48kDa

Synonyms:

IKBKG, AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9

Background:

This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.

UniProt Protein Function:	Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'-linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.
NCBI Summary:	This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. [provided by RefSeq, Mar 2016]
UniProt Code:	Q9Y6K9
NCBI GenInfo Identifier:	6685695
NCBI Gene ID:	8517
NCBI Accession:	Q9Y6K9.2
UniProt Secondary Accession:	Q9Y6K9,Q7LBY6, Q7Z7F1,
UniProt Related Accession:	Q9Y6K9
Molecular Weight:	36,953 Da
NCBI Full Name:	NF-kappa-B essential modulator
NCBI Synonym Full Names:	inhibitor of nuclear factor kappa B kinase subunit gamma
NCBI Official Symbol:	IKBKG
NCBI Official Synonym Symbols:	IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
NCBI Protein Information:	NF-kappa-B essential modulator
UniProt Protein Name:	NF-kappa-B essential modulator
UniProt Synonym Protein Names:	FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier
UniProt Gene Name:	IKBKG

	Western blot analysis of extracts of various cell lines, using IKKg antibody (CAB0917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat lung using IKKg antibody (CAB0917) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using IKKg antibody (CAB0917) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using IKKg antibody (CAB0917) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using IKKg Rabbit pAb (CAB0917) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-IKKGamma Antibody (CAB12536)[KO Validated]	Human NEMO / IKK gamma ELISA Kit
Anti-IKKGamma Antibody (CAB12561)	Human NF-kappa-B essential modulator (IKBKG) ELISA Kit
Anti-Phospho-IKKGamma-S376 Antibody (CABP1111)	IKK-gamma Colorimetric Cell-Based ELISA Kit
IKBKG Antibody (PACO00953)	IKK-gamma (Phospho-Ser31) Colorimetric Cell-Based ELISA Kit
IKBKG Antibody (PACO00954)	IKK-gamma (Phospho-Ser85) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)