The IL12B Polyclonal Antibody (CAB23054) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. RRID Gene ID 16160 Swiss Prot Synonym p40; Il-12b; Il12p40; Il-12p40; IL12B
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL12B Polyclonal Antibody
SKU:
CAB23054
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p40, Il-12b, Il12p40, Il-12p40, IL12B
Positive Sample:
HeLa, U-87 MG, 293F
Cellular Localization:
-
Calculated MW:
38kDa
Observed MW:
38kDa
This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. RRID Gene ID 16160 Swiss Prot Synonym p40; Il-12b; Il12p40; Il-12p40; IL12B
Purification Method:
Affinity purification
Gene ID:
16160
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using IL12B Rabbit pAb (CAB23054) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
