IL18 Rabbit pAb (CAB20473)

SKU:
CAB20473
  • Cell Biology Antibodies 15 IL18 Rabbit pAb CAB20473
  • Cell Biology Antibodies 15 IL18 Rabbit pAb CAB20473
  • IL18 Rabbit pAb CAB20473
  • Cell Biology Antibodies 15 IL18 Rabbit pAb CAB20473
  • IL18 Rabbit pAb CAB20473
  • Cell Biology Antibodies 15 IL18 Rabbit pAb CAB20473
  • IL18 Rabbit pAb CAB20473
€99 - €249

Description

Technical Manual

FAM129B Rabbit pAb

Product Name: IL18 Rabbit pAb (CAB20473)
Product SKU: CAB20473
Size: 20uL, 50 uL
Host Species: Rabbit
Purification Method: Affinity purification
Isotype: IgG
Background:  
Immunogen: Recombinant protein of Mouse IL18.
Sequence:  
Gene ID: 16173
Swiss Prot: P70380
Synonyms: Ig; Il-; Igif; Il-18
Calculated MW: 22kDa
Observed MW: 22kDa/18kDa
Reactivity: Human, Mouse, Rta
Application: WB, IHC
Recommended Dilution: WB 1:500 - 1:2000, IHC 1:50-1:200
Storage Buffer: Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key: Western blotting , Immunohistochemistry
Positive Samples: RAW264.7, Mouse spleen, Rat brain
Cellular Location:  

Product Images

Western blot analysis of extracts of various cell lines, using IL18 antibody (CAB20473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.

Western blot analysis of extracts of Rat brain, using IL18 antibody (CAB20473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.

Western blot analysis of extracts of Recombinant Human IL-18 Protei, using IL18 antibody (CAB20473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human tonsil using IL18 antibody (CAB20473) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using IL18 antibody (CAB20473) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat spleen using IL18 antibody (CAB20473) at dilution of 1:50 (40x lens).
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