Anti-IL-18BP Antibody (CAB6445)

Antibody Name:	IL18BP Rabbit Polyclonal Antibody
Antibody SKU:	CAB6445
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 31-194 of human IL18BP (NP_001138529.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-937, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 31-194 of human IL18BP (NP_001138529.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TPVS QTTT AATA SVRS TKDP CPSQ PPVF PAAK QCPA LEVT WPEV EVPL NGTL SLSC VACS RFPN FSIL YWLG NGSF IEHL PGRL WEGS TSRE RGST GTQL CKAL VLEQ LTPA LHST NFSC VLVD PEQV VQRH VVLA QLWA GLRA TLPP TQEA LPSS HSSP QQQG
Gene ID:	10068
Uniprot:	O95998
Cellular Location:	Secreted
Calculated MW:	12kDa/17kDa/21kDa
Observed MW:	38kDa

Synonyms:

IL18BP, IL18BPa

Background:

The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	IL18BP: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q13 Cellular Component: extracellular space; extracellular region Molecular Function:receptor antagonist activity; interleukin-18 binding Biological Process: T-helper 1 type immune response; response to lipopolysaccharide
NCBI Summary:	The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:	O95998
NCBI GenInfo Identifier:	251757286
NCBI Gene ID:	10068
NCBI Accession:	O95998.2
UniProt Secondary Accession:	O95998,O95993, O96027, Q9NZA9, Q9UBR7, B3KUZ0, B7WPK4
UniProt Related Accession:	O95998
Molecular Weight:	17,478 Da
NCBI Full Name:	Interleukin-18-binding protein
NCBI Synonym Full Names:	interleukin 18 binding protein
NCBI Official Symbol:	IL18BP
NCBI Official Synonym Symbols:	IL18BPa
NCBI Protein Information:	interleukin-18-binding protein; IL-18BP; MC51L-53L-54L homolog gene product; tadekinig-alfa
UniProt Protein Name:	Interleukin-18-binding protein
UniProt Synonym Protein Names:	Tadekinig-alfa
Protein Family:	Interleukin-18-binding protein
UniProt Gene Name:	IL18BP
UniProt Entry Name:	I18BP_HUMAN

	Western blot analysis of extracts of various cell lines, using IL18BP antibody (CAB6445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse heart using IL18BP antibody (CAB6445) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using IL18BP antibody (CAB6445). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)