Western blot analysis of various lysates, using IL4 antibody (CAB22284) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The IL4 Monoclonal Antibody (CAB22284) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated in mice, is highly specific to IL-4 and is ideal for use in various research applications, including ELISA, Western blotting, and immunohistochemistry.IL-4 is known for its role in promoting T-cell differentiation and immunoglobulin class switching in B-cells, making it a key player in adaptive immune responses. Dysregulation of IL-4 signaling has been linked to autoimmune diseases, allergies, and inflammatory disorders, highlighting the importance of studying its functions and mechanisms of action.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
IL4 Monoclonal Antibody
SKU:
CAB22284
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC56660
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Il-4, BSF-1, IL4
Positive Sample:
Recombinant Mouse IL4 protein
Cellular Localization:
External Side Of Plasma Membrane, Extracellular Space.
Calculated MW:
16kDa
Observed MW:
16kDa/18kDa(RecombinantProtein)
Enables cytokine activity. Involved in several processes, including innate immune response in mucosa; negative regulation of white fat cell proliferation; and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation; positive regulation of macromolecule metabolic process; and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain; colon; hemolymphoid system; liver; and placenta. Used to study Sjogren's syndrome; atopic dermatitis; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
Purification Method
Affinity purification
Gene ID
16189
RRID
AB_3678806
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using IL4 Rabbit mAb (CAB22284) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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