The IL6 Monoclonal Antibody (CAB22222) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, specifically targets IL6 and has been validated for use in a variety of applications including ELISA, immunofluorescence, and immunohistochemistry.IL6 is a key player in the immune system, playing a crucial role in the regulation of immune responses, inflammation, and hematopoiesis. Dysregulation of IL6 has been linked to various diseases including rheumatoid arthritis, inflammatory bowel disease, and cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL6 Monoclonal Antibody
SKU:
CAB22222
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC5093-01
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).
Purification Method
Affinity purification
Gene ID
3569
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from THP-1 cells using IL-6 Rabbit mAb (CAB22222) at 1:1000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA(80 nM) at 37℃ for overnight, LPS (1 μg/mL) and BFA (300 ng/mL) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
