Western blot analysis of various lysates, using IL7 antibody (CAB22570) at 1:30000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The IL7 Monoclonal Antibody (CAB22570) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced using cutting-edge technology, specifically targets IL7 and has been validated for use in various research applications, including ELISA, Western blot, and immunohistochemistry.IL7 is essential for the development and function of T cells, making it a crucial factor in adaptive immune responses. Dysregulation of IL7 has been linked to various autoimmune diseases, inflammatory disorders, and certain types of cancer, highlighting the importance of studying this cytokine in biomedical research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
IL7 Monoclonal Antibody
SKU:
CAB22570
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC57489
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IL-7, IL7
Positive Sample:
Recombinant Human IL-7 Protein
Cellular Localization:
Secreted.
Calculated MW:
20kDa
Observed MW:
30-35kDa
The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. IL7 plays an essential role in lymphoid cell survival, and in the maintenance of naive and memory T cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be a potent inducer of proinflammatory cytokines and chemokines which may defend against the infection, but may also mediate destructive lung injury. Elevated serum IL7 levels, together with several other circulating cytokines and chemokines, has been found to be associated with the severity of Coronavirus Disease 19 (COVID-19).
Purification Method
Affinity purification
Gene ID
3574
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using IL7 Rabbit mAb (CAB22570) at 1:30000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 120s.
