Anti-ING3 Antibody (CAB5832)

Antibody Name:	Anti-ING3 Antibody
Antibody SKU:	CAB5832
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human ING3 (NP_938008.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SW620, HeLa, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human ING3 (NP_938008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLYL EDYL EMIE QLPM DLRD RFTE MREM DLQV QNAM DQLE QRVS EFFM NAKK NKPE WREE QMAS IKKD YYKA LEDA DEKV QLAN QIYD LQHF
Gene ID:	54556
Uniprot:	Q9NXR8
Cellular Location:	Nucleus
Calculated MW:	11kDa/46kDa
Observed MW:	50kDa

Synonyms:

ING3, Eaf4, ING2, MEAF4, p47ING3

Background:

The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.

UniProt Protein Function:	ING3: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Defects in ING3 may be a cause of head and neck squamous cell carcinomas (HNSCC); also known as squamous cell carcinoma of the head and neck. Belongs to the ING family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 7q31 Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; cytoplasm; nucleolus; nucleus Molecular Function:histone acetyltransferase activity; zinc ion binding; methylated histone residue binding Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of apoptosis; regulation of growth
NCBI Summary:	The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NXR8
NCBI GenInfo Identifier:	59798432
NCBI Gene ID:	54556
NCBI Accession:	Q9NXR8.2
UniProt Related Accession:	Q9NXR8
Molecular Weight:	50kDa
NCBI Full Name:	Inhibitor of growth protein 3
NCBI Synonym Full Names:	inhibitor of growth family member 3
NCBI Official Symbol:	ING3
NCBI Official Synonym Symbols:	Eaf4; ING2; MEAF4; p47ING3
NCBI Protein Information:	inhibitor of growth protein 3
UniProt Protein Name:	Inhibitor of growth protein 3
UniProt Synonym Protein Names:	p47ING3
Protein Family:	Inhibitor of growth protein
UniProt Gene Name:	ING3
UniProt Entry Name:	ING3_HUMAN

	Western blot analysis of extracts of various cell lines, using ING3 antibody (CAB5832) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of A549 cells using ING3 antibody (CAB5832). Blue: DAPI for nuclear staining.

Antibodies

ING3 Antibody (PACO09954)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)