Western blot analysis of various lysates, using Integrin alpha 1 (ITGA1/CD49a) antibody (CAB22707) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Integrin alpha 1 (ITGA1/CD49a) Monoclonal Antibody (CAB22707) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated using hybridomas, exhibits high reactivity with human samples and is validated for use in various applications, including flow cytometry, immunofluorescence, and immunohistochemistry.Integrin alpha 1, also known as CD49a, is a key player in cell adhesion to collagen and laminin matrices, as well as in mediating cell migration and differentiation. Its role in physiological and pathological processes such as tissue repair, angiogenesis, and cancer metastasis makes it a valuable target for investigation in various research fields, including cell biology, oncology, and tissue engineering.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VLA1, CD49a, Integrin alpha 1 (ITGA1/CD49a)
Positive Sample:
SH-SY5Y, HuH-7
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
131kDa
Observed MW:
200kDa
This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
Purification Method
Affinity purification
Gene ID
3672
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Integrin alpha 1 (ITGA1/CD49a) Rabbit mAb (CAB22707) at 1:30000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
