Anti-ITGA6 Antibody (CAB3236)

Antibody Name:	Anti-ITGA6 Antibody
Antibody SKU:	CAB3236
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 870-1010 of human ITGA6 (NP_001073286.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, 293T, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 870-1010 of human ITGA6 (NP_001073286.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVES KGLE KVTC EPQK EINS LNLT ESHN SRKK REIT EKQI DDNR KFSL FAER KYQT LNCS VNVN CVNI RCPL RGLD SKAS LILR SRLW NSTF LEEY SKLN YLDI LMRA FIDV TAAA ENIR LPNA GTQV RVTV FPSK TVAQ Y
Gene ID:	3655
Uniprot:	P23229
Cellular Location:	Cell membrane, Lipid-anchor, Single-pass type I membrane protein
Calculated MW:	106kDa/119kDa/121kDa/122kDa/124kDa/126kDa
Observed MW:	105kDa

Synonyms:

CD49f, ITGA6B, VLA-6, Integrin alpha 6, ITGA6

Background:

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Cell adhesion Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: cell surface; focal adhesion; plasma membrane Molecular Function:insulin-like growth factor I binding; protein binding Biological Process: cell-substrate adhesion; cell-substrate junction assembly; extracellular matrix organization and biogenesis; gut development; hemidesmosome assembly; leukocyte migration; positive regulation of apoptosis; positive regulation of GTPase activity; positive regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; skin development Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia
NCBI Summary:	The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 6 subunit. This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. The alpha 6 beta 4 integrin may promote tumorigenesis, while the alpha 6 beta 1 integrin may negatively regulate erbB2/HER2 signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:	P23229
NCBI GenInfo Identifier:	519668687
NCBI Gene ID:	3655
NCBI Accession:	P23229.5
UniProt Secondary Accession:	P23229,Q08443, Q0MRC7, Q14646, Q16508, Q53RX7, Q59HB7 B2RMU9, B4DG69, B4DKB8, C4AM96, G5E9H1,
UniProt Related Accession:	P23229
Molecular Weight:	122,358 Da
NCBI Full Name:	Integrin alpha-6
NCBI Synonym Full Names:	integrin subunit alpha 6
NCBI Official Symbol:	ITGA6
NCBI Official Synonym Symbols:	CD49f; VLA-6; ITGA6B
NCBI Protein Information:	integrin alpha-6
UniProt Protein Name:	Integrin alpha-6
UniProt Synonym Protein Names:	CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f
Protein Family:	Integrin
UniProt Gene Name:	ITGA6
UniProt Entry Name:	ITA6_HUMAN

	Western blot analysis of extracts of various cell lines, using ITGA6 antibody (CAB3236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using ITGA6 antibody (CAB3236) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-Integrin alpha 6 Antibody (CAB19070)

Cleaved-ITGA6 (E942) Antibody (PACO00048)

ITGA6 Antibody (PACO06888)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)