Anti-IL-8 Antibody (CAB2541)

Antibody Name:	IL8 Rabbit Polyclonal Antibody
Antibody SKU:	CAB2541
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-99 of human IL8 (NP_000575.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	Recombinant Human IL-8 Protein, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-99 of human IL8 (NP_000575.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EGAV LPRS AKEL RCQC IKTY SKPF HPKF IKEL RVIE SGPH CANT EIIV KLSD GREL CLDP KENW VQRV VEKF LKRA ENS
Gene ID:	3576
Uniprot:	P10145
Cellular Location:	Secreted
Calculated MW:	11kDa
Observed MW:	11KDa

Synonyms:

CXCL8, GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1

Background:

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.

UniProt Protein Function:	IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Cytokine; Secreted; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q13-q21 Cellular Component: extracellular space; extracellular region Molecular Function:protein binding; chemokine activity; interleukin-8 receptor binding Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; unfolded protein response; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; response to molecule of bacterial origin; receptor internalization; immune response; angiogenesis; cell cycle arrest; cell motility; inflammatory response; embryonic gut development
NCBI Summary:	The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
UniProt Code:	P10145
NCBI GenInfo Identifier:	124359
NCBI Gene ID:	3576
NCBI Accession:	P10145.1
UniProt Secondary Accession:	P10145,Q6FGF6, Q6LAE6, Q96RG6, Q9C077, Q9UCE1, Q9UCR8 Q9UCR9, Q9UCS0, B2R4L8,
UniProt Related Accession:	P10145
Molecular Weight:	11,338 Da
NCBI Full Name:	Interleukin-8
NCBI Synonym Full Names:	chemokine (C-X-C motif) ligand 8
NCBI Official Symbol:	CXCL8
NCBI Official Synonym Symbols:	IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
NCBI Protein Information:	interleukin-8; emoctakin; interleukin 8; T-cell chemotactic factor; neutrophil-activating peptide 1; beta-thromboglobulin-like protein; granulocyte chemotactic protein 1; tumor necrosis factor-induced gene 1; alveolar macrophage chemotactic factor I; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; small inducible cytokine subfamily B, member 8; lymphocyte derived neutrophil activating peptide; lung giant cell carcinoma-derived chemotactic protein; beta endothelial cell-derived neutrophil activating peptide
UniProt Protein Name:	Interleukin-8
UniProt Synonym Protein Names:	C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-aAlternative name(s):GCP/IL-8 protein IV; IL8/NAP1 form I
UniProt Gene Name:	CXCL8
UniProt Entry Name:	IL8_HUMAN

	Western blot analysis of extracts of Rat lung, using IL8 antibody (CAB2541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Western blot analysis of extracts of Recombinant Human IL-8 Protein, using IL8 antibody (CAB2541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human appendix using IL8 Rabbit pAb (CAB2541) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-IL-8 Antibody (CAB12452)	Human IL-8 CLIA Kit (HUES00047)
Anti-IL-8 Antibody (CAB1289)	Human IL-8 ELISA Kit (HUEB0029)
CXCL8 Antibody (PACO00964)
CXCL8 Antibody (PACO01867)
CXCL8 Antibody (PACO04153)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)