The INTS4 Antibody (CAB6339) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is optimized for use in Western blot applications. By specifically binding to the INTS4 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a versatile tool for studies in molecular biology and gene regulation.INTS4 plays a crucial role in transcriptional regulation by facilitating the processing of small nuclear RNAs and promoting mRNA formation.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
INTS4 Antibody
SKU:
CAB6339
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INT4, MST093, INTS4
Positive Sample:
HepG2, Jurkat, Mouse testis, Rat liver
Cellular Localization:
Nucleus.
Calculated MW:
108kDa
Observed MW:
108kDa
INTS4 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).
Purification Method
Affinity purification
Gene ID
92105
RRID
AB_2766942
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using INTS4 Rabbit pAb (CAB6339) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Rat liver, using INTS4 Rabbit pAb (CAB6339) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
