Anti-INTS6 Antibody (CAB6634)

Antibody Name:	Anti-INTS6 Antibody
Antibody SKU:	CAB6634
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human INTS6 (NP_036273.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HL-60, SW620, HeLa, NCI-H460, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human INTS6 (NP_036273.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPIL LFLI DTSA SMNQ RSHL GTTY LDTA KGAV ETFM KLRA RDPA SRGD RYML VTFE EPPY AIKA GWKE NHAT FMNE LKNL QAEG LTTL GQSL RTAF DLLN LNRL VTGI DNYG QGRN PFFL EPAI IITI TDGS KLTT TSGV QDEL HLPL NSPL PGSE LTKE PFRW DQRL FALV LRLP GTMS VESE QLTG VPLD DSAI TPMC EVTG GRSY SVCS PRML NQCL ESLV QKVQ SGVV INFE KAGP DPSP VEDG QPDI SRPF GSQP WHSC HKLI YVRP NPKT GVPI
Gene ID:	26512
Uniprot:	Q9UL03
Cellular Location:	Nucleus
Calculated MW:	12kDa/98kDa/100kDa
Observed MW:	115kDa

Synonyms:

INTS6, DBI-1, DDX26, DDX26A, DICE1, HDB, INT6, Notchl2

Background:

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and is located in the critical region of loss of heterozygosity (LOH). Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	INTS6: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. May have a tumor suppressor role; an ectopic expression suppressing tumor cell growth. Belongs to the Integrator subunit 6 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA processing; Receptor, misc. Chromosomal Location of Human Ortholog: 13q14.3 Cellular Component: nucleoplasm; integrator complex; actin cytoskeleton Molecular Function:protein binding; transmembrane receptor activity Biological Process: snRNA processing; signal transduction
NCBI Summary:	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and located in the critical region of loss of heterozygosity (LOH). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UL03
NCBI GenInfo Identifier:	74753376
NCBI Gene ID:	26512
NCBI Accession:	Q9UL03.1
UniProt Secondary Accession:	Q9UL03,Q0P664, Q6PJP4, Q9UFK0, Q9Y5M9,
UniProt Related Accession:	Q9UL03
Molecular Weight:	887
NCBI Full Name:	Integrator complex subunit 6
NCBI Synonym Full Names:	integrator complex subunit 6
NCBI Official Symbol:	INTS6
NCBI Official Synonym Symbols:	HDB; INT6; DBI-1; DDX26; DICE1; DDX26A; Notchl2
NCBI Protein Information:	integrator complex subunit 6; DEAD box protein; RNA helicase HDB; protein deleted in cancer 1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26
UniProt Protein Name:	Integrator complex subunit 6
UniProt Synonym Protein Names:	DBI-1; Protein DDX26; Protein deleted in cancer 1; DICE1
Protein Family:	Integrator complex
UniProt Gene Name:	INTS6
UniProt Entry Name:	INT6_HUMAN

	Western blot analysis of extracts of various cell lines, using INTS6 antibody (CAB6634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody (CAB6634) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using INTS6 Antibody (CAB6634) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using INTS6 antibody (CAB6634). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)