Anti-ITGB3BP Antibody (CAB14859)

Antibody Name:	Anti-ITGB3BP Antibody
Antibody SKU:	CAB14859
Antibody Size:	20uL, 50uL, 100uL
Application:	IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-111 of human ITGB3BP (NP_055103.3).

Application:	IHC
Recommended Dilution:	IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-111 of human ITGB3BP (NP_055103.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPVK RSLK LDGL LEEN SFDP SKIT RKKS VITY SPTT GTCQ MSLF ASPT SSEE QKHR NGLS NEKR KKLN HPSL TESK ESTT KDND EFMM LLSK VEKL SEEI MEIM QNLS SIQ
Gene ID:	23421
Uniprot:	Q13352
Cellular Location:	Chromosome, Cytoplasm, Cytoplasm, Nucleus, centromere, kinetochore
Calculated MW:	6kDa/12kDa/19kDa/20kDa/24kDa
Observed MW:

Synonyms:

ITGB3BP, CENP-R, CENPR, HSU37139, NRIF3, TAP20

Background:

This gene encodes a transcriptional coregulator that binds to and enhances the activity of members of the nuclear receptor families, thyroid hormone receptors and retinoid X receptors. This protein also acts as a corepressor of NF-kappaB-dependent signaling. This protein induces apoptosis in breast cancer cells through a caspase 2-mediated signaling pathway. This protein is also a component of the centromere-specific histone H3 variant nucleosome associated complex (CENP-NAC) and may be involved in mitotic progression by recruiting the histone H3 variant CENP-A to the centromere. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	NRIF3: Transcription coregulator that can have both coactivator and corepressor functions. Isoform 1, but not other isoforms, is involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF- kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A- associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Homodimer; mediated by the coiled coil domain. Isoform 3, but not other isoforms, interacts with the cytoplasmic tail of integrin ITGB3. The relevance of the interaction with ITGB3 is however uncertain, since isoform 3 is mainly nuclear. Interacts with CCNA2 and MTA1. Interacts with NFKB1 NF-kappa-B subunit. Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. By estrogen. Widely expressed. Expressed in spleen, thymus, prostate, ovary, small intestine and white blood cells. Highly expressed in testis and colon. Isoform 4 is expressed in platelets, lymphocytes and granulocytes. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Cell adhesion; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p31.3 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; cytosol Molecular Function:protein C-terminus binding; signal transducer activity Biological Process: mitosis; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; nerve growth factor receptor signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of apoptosis; cell division; mitotic cell cycle; cell adhesion; signal transduction
NCBI Summary:	This gene encodes a transcriptional coregulator that binds to and enhances the activity of members of the nuclear receptor families, thyroid hormone receptors and retinoid X receptors. This protein also acts as a corepressor of NF-kappaB-dependent signaling. This protein induces apoptosis in breast cancer cells through a caspase 2-mediated signaling pathway. This protein is also a component of the centromere-specific histone H3 variant nucleosome associated complex (CENP-NAC) and may be involved in mitotic progression by recruiting the histone H3 variant CENP-A to the centromere. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
UniProt Code:	Q13352
NCBI GenInfo Identifier:	50400855
NCBI Gene ID:	23421
NCBI Accession:	Q13352.2
UniProt Secondary Accession:	Q13352,Q9CQ82, Q5U1Z7,
UniProt Related Accession:	Q13352
Molecular Weight:	177
NCBI Full Name:	Centromere protein R
NCBI Synonym Full Names:	integrin beta 3 binding protein (beta3-endonexin)
NCBI Official Symbol:	ITGB3BP
NCBI Official Synonym Symbols:	CENPR; NRIF3; TAP20; CENP-R; HSU37139
NCBI Protein Information:	centromere protein R; beta 3 endonexin; beta-3-endonexin; integrin beta-3-binding protein; nuclear receptor-interacting factor 3
UniProt Protein Name:	Centromere protein R
UniProt Synonym Protein Names:	Beta-3-endonexin; Integrin beta-3-binding protein; Nuclear receptor-interacting factor 3
UniProt Gene Name:	ITGB3BP
UniProt Entry Name:	CENPR_HUMAN

	Immunohistochemistry of paraffin-embedded human liver using ITGB3BP antibody (CAB14859) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using ITGB3BP antibody (CAB14859) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using ITGB3BP antibody (CAB14859) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ITGB3BP antibody (CAB14859) at dilution of 1:100 (40x lens).

Antibodies

Anti-ITGB3BP Antibody (CAB5839)

ITGB3BP Antibody (PACO10010)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)