Description
system_update_altDatasheet
| Product Name: | JC-10 Mitochondrial Membrane Potential Assay Kit |
| Product Code: | ARMA00351 |
| Detection Principle: | Ratiometric Fluorescence Assay |
| Monomer Channel (Green): | Ex 490 nm / Em 525 nm (Depolarised Mitochondria) |
| Aggregate Channel (Orange/Red): | Ex 540 nm / Em 590 nm (Polarised Mitochondria) |
| Reading Mode: | Bottom-Read, Black-walled clear-bottom 96-well plate |
JC-10 is a dual-emission ratiometric probe that measures mitochondrial membrane potential. In healthy cells with high mitochondrial membrane potential, JC-10 concentrates inside mitochondria and forms J-aggregates, emitting orange fluorescence (540 nm / 590 nm). In stressed or depolarised cells where membrane potential collapses, JC-10 disperses into the cytoplasm as monomers, shifting fluorescence to green (490 nm / 525 nm). The ratio of orange-to-green fluorescence (F590 / F525) directly measures mitochondrial depolarisation.
| Cell Seeding: | Black-walled, clear-bottom 96-well plate; 100 µl final volume per well (80 µl medium + 20 µl test compound) |
| Positive Control (FCCP): | Add 10 µl of 40 µM FCCP to designated wells; incubate 30 min at 37°C |
| Dye Loading: | Add 50 µl JC-10 Dye Loading Solution to all wells (no medium removal required) |
| Dye Incubation: | 30-60 minutes at 37°C, 5% CO₂; protect from direct light |
| Masking: | Add 50 µl Masking Solution to all wells; do not remove liquid |
| Measurement: | Immediate ratiometric measurement (F590 / F525) in bottom-read mode |
| Unopened Kit Storage: | -20°C |
| JC-10 Dye (Reconstituted): | -20°C; Reconstitute with 245 µl anhydrous DMSO |
| FCCP Control (Reconstituted): | -20°C; Reconstitute with 50 µl anhydrous DMSO |
| Dye Loading Buffer: | 4°C; Ready to use |
| Masking Buffer: | 4°C; Ready to use |
| All JC-10 Solutions: | Protect from direct light |
