KChIP3 Antibody [X12] is a rabbit recombinant monoclonal antibody directed against KChIP3, offered by Assay Genie for research applications. Reported applications include WB. Reported reactivity: Human, Mouse and Rat. Supplied as IgG Kappa, purified by AmMag™ Ultra AT Protein A MagBeads.
KChIP3, also known as DREAM or calsenilin, is a neuronal calcium-binding protein that plays a central role in regulating gene expression, potassium channel activity, and neurodegenerative signaling. It modulates Kv4 potassium channels, thereby influencing neuronal excitability and synaptic plasticity, which are key processes disrupted in neurodegenerative diseases such as Alzheimer’s. In the nucleus, KChIP3 acts as a calcium-dependent transcriptional repressor, regulating genes like prodynorphin that are involved in pain signaling and neuroprotection. Its interaction with presenilin proteins affects amyloid precursor protein processing, linking KChIP3 to Alzheimer’s disease pathology. Our recombinant monoclonal antibody targeting rat KChIP3 has been shown to enable high-specificity detection in rodent brain lysates and in overexpression lysates This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
1. Wang, S. et al. (2002). Kinetic properties of Kv4.3 and their modulation by KChIP2b. Biochem Biophys Res Commun, 295(2), 223-229. https://doi.org/10.1016/S0006-291X(02)00658-7 2. Van Vickle, G.D., Esh, C.L., Kokjohn, T.A. et al. Presenilin-1 280Glu→Ala Mutation Alters C-Terminal APP Processing Yielding Longer Aβ Peptides: Implications for Alzheimer’s Disease. Mol Med 14, 184–194 (2008). https://doi.org/10.2119/2007-00094.VanVickle 3. Zaidi, N. F., Kuplast, K. G., Washicosky, K. J., Kajiwara, Y., Buxbaum, J. D., & Wasco, W. (2006). Calsenilin interacts with transcriptional co-repressor C-terminal binding protein(s). Journal of neurochemistry, 98(4), 1290–1301. https://doi.org/10.1111/j.1471-4159.2006.03972.x
![Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Human KChIP3 protein in a transient over-expression lysate (OriGene LY415545). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279999/766257/anti-kchip3-antibody-x12__45632.1785527591.jpg?c=2?imbypass=on "Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Human KChIP3 protein in a transient over-expression lysate (OriGene LY415545). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
![Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Rat KChIP3 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279999/766458/anti-kchip3-antibody-x12__09538.1785528200.jpg?c=2?imbypass=on "Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Rat KChIP3 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
![Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Mouse KChIP3 protein in a Mouse Brain lysate. Block: 5% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/1280x1280/products/279999/761145/anti-kchip3-antibody-x12__40597.1785511424.jpg?c=2?imbypass=on "Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Mouse KChIP3 protein in a Mouse Brain lysate. Block: 5% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
![Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Human KChIP3 protein in a transient over-expression lysate (OriGene LY415545). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/608x608/products/279999/766257/anti-kchip3-antibody-x12__45632.1785527591.jpg?c=2 "Western blot analysis with Rabbit Anti-Rat KChIP3 Monoclonal Antibody, Clone X12 (STMA00233) showing detection of Human KChIP3 protein in a transient over-expression lysate (OriGene LY415545). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat KChIP3 Recombinant Monoclonal [X12] (STMA00233) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT.")
