Western blot analysis of extracts from A-549 cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (CAB22706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Monoclonal Antibody (CAB22706) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through stringent validation processes, is highly specific for human samples and is suited for Western blot and immunofluorescence applications.Integrin α5 is a key player in cell adhesion and signaling pathways, influencing processes like angiogenesis, wound healing, and cancer metastasis. By targeting Integrin α5 with this monoclonal antibody, researchers can effectively analyze its expression and function in various cell types, aiding in the investigation of diseases such as cancer, fibrosis, and cardiovascular disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Purification Method
Affinity purification
Gene ID
3678
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (CAB22706) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (CAB22706) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
![Anti- Integrin alpha 5 [R02-3G4] Monoclonal Antibody - Knockout Validated (AGMB06808)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/278089/732664/anti-integrin-alpha-5-r02-3g4-monoclonal-antibody-knockout-validated-agmb06808__86331.1777187593.jpg?c=2 "Anti- Integrin alpha 5 [R02-3G4] Monoclonal Antibody - Knockout Validated (AGMB06808)")
