Western blot analysis of extracts from wild type(WT) and ISG15 Rabbit pAb knockdown (KD) HeLa cells, using ISG15 Rabbit pAb antibody (CAB21995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The [KD Validated] ISG15 Polyclonal Antibody (CAB21995) is a high-quality antibody developed for reliable detection and analysis of target proteins. Produced by Assay Genie, this antibody is raised in rabbits and has been extensively validated for use in a variety of research applications, including Western blotting.ISG15, also known as interferon-stimulated gene 15, is a ubiquitin-like protein that plays a crucial role in the innate immune response against viral infections. By targeting proteins for degradation and modulating immune signaling pathways, ISG15 helps to eliminate infected cells and control the spread of viruses within the body.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KD Validated] ISG15 Polyclonal Antibody
SKU:
CAB21995
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Purification Method
Affinity purification
Gene ID
9636
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and ISG15 knockdown (KD) HeLa cells, using [KD Validated] ISG15 Rabbit pAb (CAB21995) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
