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Anti-KIFC1 Antibody (CAB3304)

SKU:
CAB3304
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-KIFC1 Antibody CAB3304
  • Cell Cycle Antibodies 1 Anti-KIFC1 Antibody CAB3304
  • Cell Cycle Antibodies 1 Anti-KIFC1 Antibody CAB3304
  • Cell Cycle Antibodies 1 Anti-KIFC1 Antibody CAB3304
  • Cell Cycle Antibodies 1 Anti-KIFC1 Antibody CAB3304
€139 - €419
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Description

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Antibody Name:Anti-KIFC1 Antibody
Antibody SKU:CAB3304
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HT-29, HeLa, A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human KIFC1 (NP_002254.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDPQ RSPL LEVK GNIE LKRP LIKA PSQL PLSG SRLK RRPD QMED GLEP EKKR TRGL GATT KITT SHPR VPSL TTVP QTQG QTTA QKVS KKTG PRCS TAIA TGLK NQKP VPAV PVQK SGTS GVPP MAGG KKPS KRPA WDLK GQLC DLNA ELKR CRER TQTL DQEN QQLQ DQLR DAQQ QVKA LGTE RTTL EGHL AKVQ AQAE QGQQ ELKN LRAC VLEL EERL STQE GLVQ ELQK KQVE LQEE RRGL MSQL EE
Gene ID:3833
Uniprot:Q9BW19
Cellular Location:Cytoplasm, Early endosome, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle
Calculated MW:73kDa
Observed MW:74kDa
Synonyms:KIFC1, HSET, KNSL2
Background:
UniProt Protein Function:KIFC1: Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Belongs to the kinesin-like protein family. NCD subfamily. Binds NUBP1 and NUBP2.
UniProt Protein Details:

Protein type:Motor; Microtubule-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: kinesin complex; microtubule; membrane; early endosome; microtubule organizing center; spindle; nucleus

Molecular Function:minus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; ATP binding

Biological Process: mitotic sister chromatid segregation; metabolic process; cell division; spindle assembly; blood coagulation; mitotic metaphase plate congression; microtubule-based movement

UniProt Code:Q9BW19
NCBI GenInfo Identifier:20138710
NCBI Gene ID:3833
NCBI Accession:Q9BW19.2
UniProt Secondary Accession:Q9BW19,O60887, Q14834, Q4KMP0, Q5SU09, Q6GMS7, Q6P4A5 Q9UQP7,
UniProt Related Accession:Q9BW19
Molecular Weight:74kDa
NCBI Full Name:Kinesin-like protein KIFC1
NCBI Synonym Full Names:kinesin family member C1
NCBI Official Symbol:KIFC1  
NCBI Official Synonym Symbols:HSET; KNSL2  
NCBI Protein Information:kinesin-like protein KIFC1; kinesin-like 2; kinesin-like protein 2; kinesin-related protein HSET
UniProt Protein Name:Kinesin-like protein KIFC1
UniProt Synonym Protein Names:Kinesin-like protein 2; Kinesin-related protein HSET
Protein Family:Kinesin-like protein
UniProt Gene Name:KIFC1  
UniProt Entry Name:KIFC1_HUMAN
Anti-KIFC1 Antibody (CAB3304)Western blot analysis of extracts of various cell lines, using KIFC1 antibody (CAB3304) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-KIFC1 Antibody (CAB3304)Immunohistochemistry of paraffin-embedded rat testis using KIFC1 antibody (CAB3304) at dilution of 1:100 (40x lens).
Anti-KIFC1 Antibody (CAB3304)Immunohistochemistry of paraffin-embedded human colon carcinoma using KIFC1 antibody (CAB3304) at dilution of 1:100 (40x lens).
Anti-KIFC1 Antibody (CAB3304)Immunohistochemistry of paraffin-embedded mouse testis using KIFC1 antibody (CAB3304) at dilution of 1:100 (40x lens).
Anti-KIFC1 Antibody (CAB3304)Immunofluorescence analysis of U-2 OS cells using KIFC1 Polyclonal Antibody (CAB3304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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