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KIT Rabbit Polyclonal Antibody (CAB7521)

SKU:
CAB7521
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:KIT Rabbit Polyclonal Antibody
SKU:CAB7521
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 867-976 of human CD117/c-Kit (NP_000213.1).
Sequence:SSPY PGMP VDSK FYKM IKEG FRML SPEH APAE MYDI MKTC WDAD PLKR PTFK QIVQ LIEK QISE STNH IYSN LANC SPNR QKPV VDHS VRIN SVGS TASS SQPL LVHD DV
Tested Applications:WB ELISA
Recommended Dilution:WB,1:1000 - 1:5000
Synonyms:PBT; SCFR; C-Kit; CD117; MASTC; CD117/c-Kit
Positive Sample:Mouse lung,Mouse brain
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Single-pass type I membrane protein.
Calculated MW:110kDa
Observed MW:120kDa/145kDa

This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:3815
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using CD117/c-Kit antibody (CAB7521) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.

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