The KLHDC2 Antibody (CAB15146) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the KLHDC2 protein, this antibody allows for the detection and analysis of KLHDC2 in a variety of cell types, making it an ideal choice for studies in cell biology and molecular biology.KLHDC2 is a protein involved in protein degradation pathways and has been linked to the regulation of cell cycle progression and cell growth.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
KLHDC2 Antibody
SKU:
CAB15146
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LCP, HCLP1, HCLP-1, KLHDC2
Positive Sample:
K-562
Cellular Localization:
Nucleus.
Calculated MW:
46kDa
Observed MW:
46kDa
Enables ubiquitin ligase-substrate adaptor activity. Involved in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway. Located in nuclear body and nuclear membrane. Is active in Cul2-RING ubiquitin ligase complex and nucleus.
Purification Method
Affinity purification
Gene ID
23588
RRID
AB_2762033
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using KLHDC2 Rabbit pAb (CAB15146) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 150s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using KLHDC2 Rabbit pAb (CAB15146) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
