Anti-CALR Antibody (CAB18013)

Antibody Name:	Anti-CALR Antibody [KO Validated]
Antibody SKU:	CAB18013
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-417 of human CALR (NP_004334.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NIH/3T3, SH-SY5Y, Mouse liver, Rat liver, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 18-417 of human CALR (NP_004334.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EPAV YFKE QFLD GDGW TSRW IESK HKSD FGKF VLSS GKFY GDEE KDKG LQTS QDAR FYAL SASF EPFS NKGQ TLVV QFTV KHEQ NIDC GGGY VKLF PNSL DQTD MHGD SEYN IMFG PDIC GPGT KKVH VIFN YKGK NVLI NKDI RCKD DEFT HLYT LIVR PDNT YEVK IDNS QVES GSLE DDWD FLPP KKIK DPDA SKPE DWDE RAKI DDPT DSKP EDWD KPEH IPDP DAKK PEDW DEEM DGEW EPPV IQNP EYKG EWKP RQID NPDY KGTW IHPE IDNP EYSP DPSI YAYD NFGV LGLD LWQV KSGT IFDN FLIT NDEA YAEE FGNE TWGV TKAA EKQM KDKQ DEEQ RLKE EEED KKRK EEEE AEDK EDDE DKDE DEED EEDK EEDE EEDV PGQA KDEL
Gene ID:	811
Uniprot:	P27797
Cellular Location:	Cell surface, Cytoplasm, Endoplasmic reticulum lumen, Sarcoplasmic reticulum lumen, Secreted, cytosol, extracellular matrix, extracellular space
Calculated MW:	48kDa
Observed MW:	55KDa

	Western blot analysis of extracts from normal (control) and CALR knockout (KO) HeLa cells, using CALR antibody (CAB18013) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using CALR antibody (CAB18013) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using [KO Validated] CALR Rabbit pAb (CAB18013) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] CALR Rabbit pAb (CAB18013) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] CALR Rabbit pAb (CAB18013) at dilution of 1:100 (40x lens).

Synonyms:

CALR, CRT, HEL-S-99n, RO, SSA, cC1qR

Background:

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

UniProt Protein Function:	Calreticulin: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57. Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Belongs to the calreticulin family.
UniProt Protein Details:	Protein type:Calcium-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19p13.13 Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; extracellular space; focal adhesion; intracellular; membrane; nucleus; perinuclear region of cytoplasm; polysome Molecular Function:androgen receptor binding; calcium ion binding; carbohydrate binding; chaperone binding; complement component C1q binding; glycoprotein binding; integrin binding; mRNA binding; protein binding; ubiquitin protein ligase binding; unfolded protein binding; zinc ion binding Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular calcium ion homeostasis; glucocorticoid receptor signaling pathway; negative regulation of neuron differentiation; negative regulation of retinoic acid receptor signaling pathway; negative regulation of steroid hormone receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of translation; peptide antigen assembly with MHC class I protein complex; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of phagocytosis; protein export from nucleus; protein folding; protein maturation via protein folding; protein stabilization; receptor-mediated endocytosis; regulation of apoptosis; regulation of transcription, DNA-dependent; sequestering of calcium ion Disease: Myelofibrosis; Thrombocythemia 1
NCBI Summary:	Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]
UniProt Code:	P27797
NCBI GenInfo Identifier:	117501
NCBI Gene ID:	811
NCBI Accession:	P27797.1
UniProt Secondary Accession:	P27797,Q6IAT4, Q9UDG2,
UniProt Related Accession:	P27797
Molecular Weight:	48,142 Da
NCBI Full Name:	Calreticulin
NCBI Synonym Full Names:	calreticulin
NCBI Official Symbol:	CALR
NCBI Official Synonym Symbols:	RO; CRT; SSA; cC1qR; HEL-S-99n
NCBI Protein Information:	calreticulin
UniProt Protein Name:	Calreticulin
UniProt Synonym Protein Names:	CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60
Protein Family:	Calreticulin
UniProt Gene Name:	CALR

Antibodies	ELISA Kits
Anti-CALR Antibody (CAB1066)	Human CRT / Calreticulin ELISA Kit
Anti-Calreticulin Antibody (CAB11563)	Human CRT (Calreticulin) CLIA Kit (HUES00414)
CALR Antibody (PACO00504)	Human CRT (Calreticulin) ELISA Kit (HUES01790)
	Human Calreticulin (CALR) ELISA Kit
	CALR Colorimetric Cell-Based ELISA

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)