Anti-CD98 Antibody (CAB19880)[KO Validated]

SKU:
CAB19880
  • KO Validated Antibodies 2 Anti-CD98 Antibody CAB19880KO Validated
  • KO Validated Antibodies 2 Anti-CD98 Antibody CAB19880KO Validated
  • KO Validated Antibodies 2 Anti-CD98 Antibody CAB19880KO Validated
  • KO Validated Antibodies 2 Anti-CD98 Antibody CAB19880KO Validated
  • KO Validated Antibodies 2 Anti-CD98 Antibody CAB19880KO Validated
€129 - €349

Description

Technical Manual

Anti-CD98 Antibody [KO Validated]

Product Name:Anti-CD98 Antibody [KO Validated]
Product SKU:CAB19880
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.

Immunogen:Recombinant protein of human CD98.
Sequence:

Email for sequence

Gene ID:6520
Swiss Prot:P08195
Synonyms:SLC3A2; 4F2; 4F2HC; 4T2HC; CD98; CD98HC; MDU1; NACAE
Calculated MW:57kDa/61kDa/67kDa/71kDa
Observed MW:75-100KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:HeLa, Raji, SH-SY5Y, Rat kidney
Cellular Location:Apical cell membrane, Melanosome, Single-pass type II membrane protein

Product Images

Anti-CD98 Antibody [KO Validated] (CAB19880)

Western blot analysis of extracts from normal (control) and CD98 knockout (KO) HeLa cells, using CD98 antibody (CAB19880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10S.

Anti-CD98 Antibody [KO Validated] (CAB19880)

Western blot analysis of extracts of various cell lines, using CD98 antibody (CAB19880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Anti-CD98 Antibody [KO Validated] (CAB19880)

Western blot analysis of extracts of Rat kidney, using CD98 antibody (CAB19880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.

Anti-CD98 Antibody [KO Validated] (CAB19880)

Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] CD98 Rabbit pAb (CAB19880) at dilution of 1:100 (40x lens).

Anti-CD98 Antibody [KO Validated] (CAB19880)

Immunohistochemistry of paraffin-embedded human placenta using [KO Validated] CD98 Rabbit pAb (CAB19880) at dilution of 1:100 (40x lens).

Uniprot Information

UniProt Protein Function:Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567).
NCBI Summary:This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. [provided by RefSeq, Nov 2010]
UniProt Code:P08195
NCBI GenInfo Identifier:257051063
NCBI Gene ID:6520
NCBI Accession:P08195.3
UniProt Related Accession:P08195
Molecular Weight:
NCBI Full Name:4F2 cell-surface antigen heavy chain
NCBI Synonym Full Names:solute carrier family 3 member 2
NCBI Official Symbol:SLC3A2 
NCBI Official Synonym Symbols:4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC 
NCBI Protein Information:4F2 cell-surface antigen heavy chain
UniProt Protein Name:4F2 cell-surface antigen heavy chain
UniProt Synonym Protein Names:4F2 heavy chain antigen; Lymphocyte activation antigen 4F2 large subunit; Solute carrier family 3 member 2; CD_antigen: CD98
UniProt Gene Name:SLC3A2 
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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